Biological control of some types of mycotoxins by probiotic bacteria and yeast strains

Ab EL- Fadeel, Reda Hussein; Ahmed Shalaby, Samir Okasha; Arafa Mohamed, Mahmod Mohamed; Khater, Khater Abd-El Fattah

doi:10.21608/ejds.2022.171199.1008

Biological control of some types of mycotoxins by probiotic bacteria and yeast strains

Document Type : Original Article

Authors

¹ Dairy Department, AL-AZhar Faculty of Agriculture, 1 permanent camp, Al-Azhar University, Nasr City, Cairo Governorate, Nasr City, Cairo Egypt

² Biochemistry Toxicology and Nutritionals Differences Disses Dept. AHRI- ARC.

³ Department of Dairy, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

10.21608/ejds.2022.171199.1008

Abstract

Fungal and mycotoxin contamination of raw milk and dairy products are constitute a potential hazard to human health and food safety. Therefore, the present study was designed to assess the potential use of antifungal compounds produced by probiotic bacteria and yeast strains for growth inhibition and subsequent aflatoxin (AFS) and ochratoxin (OTA) production from select strains of Aspergillus flavus (OK605586) and Penicillium nordicum (OK605551), respectively. The AFS and OTA residues were determined by Leteral Flow Immuno Assay (LFIA) after 14 days of incubation for strains, at 25 °C in YES medium, as a model for mycotoxins determination. According to the results of the current investigation, probiotic bacteria and yeast strains had good ability to inhibit growth of toxigenic fungi strains. There were noticeable decreases in mycotoxins production, as well L. plantarum exhibited the highest reduction figures of AFS and OTA production by A. flavus and P. nordicum, being 64.51% and 87.21%, respectively. Moreover, the combination of L. acidophilus and L. plantarum succeeded to reduce AFS and OTS concentrations from 35 ng/ml and 7.82 ng/ml to zero ng/ml, for both toxins after 14 days of incubation, with the highest reduction value, actually 100%. However, the same trend of result was also observed in case of probiotic yeasts, where S. cerevisiae showed the best ability to reduce AFS and OTA concentrations by 83.15% and 75.6% respectively, as compared with T. delbrueckii strain. Additionally, the combination of S.cerevisiae and T. delbrueckii demonstrated the greatest reduction figure in AFS and OTA concentration, actually 100%.

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